Cytometry Library: protocols, tutorials, user manuals.

General Cytometry Information

Dako - Guide to Flow Cytometry (PDF)
Basic introductory guide to flow cytometry. We highly recommend looking it over!

Practical Flow Cytometry, Fourth Edition
Free downloadable version of Howard Shapiro's comprehensive textbook.

Wikipedia: Flow cytometry
Wikipedia is an online encyclopedia. Link goes to their entry on flow cytometry.

How a Flow Cytometer Works
A brief introduction to the history, science, and engineering behind flow cytometry from Science Park at the University of Texas MD Anderson Cancer Center.

BD Customer Training Tools
Tutorials and Quick Reference Guides to the software used with the LSR II, FACSCalibur, and the FACSCalibur HTS unit.

FlowJo - Analysis Software

FlowJo is very powerful flow cytometry analysis software you can download for free but requires a license to run. We offer several dongles you may borrow and run FlowJo on your own computer, as well as have several computers in the lab with FlowJo installed. To borrow a dongle, stop by the flow lab.

FlowJo Tech Notes
Four page introductory pamphlets: Getting Started, Compensation, Kinetics, Cell Cycle

FlowJo Tutorials and Demo Data
In-depth tutorials with demo FCS data sets. Also tutorial videos.

FlowJo Online Manuals
Manuals for Windows and Macintosh, printable, for multiple versions.

Colors and Compensation

Mario Roederer's Compensation Perspective
Excellent guide to compensation, how to do it, and why it's so important. Includes a short no-fail recipe for proper compensation every time and a quiz to test your knowledge.

Table of Fluorochromes
Table of common fluorochromes from the Salk Center for Cytometry. Includes excitation and emission wavelengths, MW, and some notes on use.

BD Fluorescence Spectrum Viewer
Viewer provides spectral visualization of excitation and emission peaks for many common fluorochromes.

Invitrogen Fluorescence SpectraViewer
Viewer provides spectral visualization of excitation and emission peaks for many common fluorochromes.

The fluorescent toolbox for assessing protein location and function.
Giepmans BN, Adams SR, Ellisman MH, Tsien RY. Science. 2006 Apr 14;312(5771):217-24.

Protocols, Papers, and Publications

BD Pharmingen Protocols
Isotype selection, cell surface staing of PBMCs, Annexin V Staining, BRDU incorporation, Ki-67 Detection, and more.

Cell Cycle Analysis - DNA Content
DNA content protocol hosted at MD Anderson Cancer Center

Out of the blue: a comparison of Hoechst side population (SP) analysis of murine bone marrow using 325, 363 and 407 nm excitation sources.
Simpson C, Pearce DJ, Bonnet D, Davies D. J Immunol Methods. 2006 Mar 20;310(1-2):171-81. Epub 2006 Feb 7.

Side Population Analysis Using a Violet-Excited Cell-Permeable DNA Binding Dye
Telford W, Bradford J, Godfrey W, Robey R, Bates S. Stem Cells Vol. 25 No. 4 April 2007, pp. 1029-1036
(Uses the cell permeant DNA stain Vybrant DyeCycle Violet from Invitrogen.)

Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells
Hideo Ema, Yohei Morita, Hiromitsu Nakauchi, and Yumi Matsuzaki. Current Protocols in Immunology (2005) 22B..1.1-22B.1.13 John Wiley & Sons.